Martes, 21 de enero de 2025

El Dr. Miguel Holgado (Responsable del Grupo de Investigación Organ and Tissue on-a-chip and In-Vitro Detection del IdISSC) presenta los resultados específicos que están desarrollando en oftalmología en un seminario el próximo martes 21 de enero a las 14:00 h en el aula Fernández Cruz del Hospital y ver oportunidades de colaboración.

Os hacemos llegar la información de la investigación que se tratará en el seminario:

Human cornea ex vivo model on a chip for real time monitoring infection of Aspergillus fumigatus and Candida albicans and the effect of amphotericin B, and advanced biosensing system for fungal and virus detection in cornea.

Miguel Holgado.

Full Professor/Catedrático de la Universidad Politécnica de Madrid (UPM). Director of Optics, Photonics and Biophotonics Group (GOFB-UPM)  at the Center for Biomedical technology (CTB-UPM). Group Leader of Organ and tissue on a Chip and in vitro detection at the Health Research Institute of the Hospital Clínico San Carlos (IDISSC) and  Professor of the Department of Applied Physics and Materials Engineering at the Industrial Engineering Faculty (ETSII-UPM).

Abstract

Organs-on-chips is a powerful technology for in vitro monitoring of human pathologies and the effect of therapeutics. This technology is based on continuous media flow chamber systems containing small-scale tissues to better mimic human physiology with direct application for personalized medicine and discovering of new drugs. On the other hand, biomarkers screening technologies are of great importance, especially when they are reliable, cost-effective, easy to use to measure multiple biomarkers in a single disposable diagnostic KIT that is capable of working with real biological samples such as tears, serum, saliva, wastewater or culture medium. It would be a major breakthrough if in a single diagnostic KIT it were possible to specifically detect bacterial, viral, fungal and parasitic infection. There is currently no in vitro diagnostic system capable of doing this based on immunoassay techniques in a relatively short time.

In this paper we report a human ex vivo model of Aspergillus fumigatus and Candida albicans infection of a cornea on-a-chip to understand corneal biological reaction focusing on improving tissue preservation and monitoring the fungal infection. As a result, we demonstrate the ability to monitor fungal behavior and growth on the cornea and the effect of amphotericin B as therapy. Additionally, we establish a method for collection of fungal antigens in polyethersulfone membrane-based sample collectors from the surface of fungal inoculated corneas and devised an original ad-hoc method to monitor fungi in corneal tissue as an alternative to microbiological culture. Finally, we present a novel approach based on a new technology capable of detecting multiple biomarkers in a way that integrates multiple biosensors in a single disposable diagnostic KIT, and thus will be able to identify biological agents in eye infections using simple immunoassay technology. We show the results for the detection of fungi and viruses.

Acknowledgements

• Sarp Orgul, Darlene Miller, Alfonso Sabater and Angela Gómez.
• Bascom Palmer Eye Institute. University of Miami Health System. USA.
• Instituto de Salud Carlos III under the project PT23/00167,
• Madrid Regional Research Program under the Project IND2023/BMD-28229
• Universidad Politécnica de Madrid Mobility Program.

Modalidad presencial: Hospital Clínico Universitario San Carlos. Aula Fernández-Cruz (Pabellón docente)

Correo de contacto: fibgestor.hcsc@salud.madrid.org