Flow Cytometry Unit (FCU)

Flow Cytometry Unit

The Transversal Flow Cytometry Unit of the Health Research Institute (Instituto de Investigación Sanitaria) of the Hospital Clínico San Carlos (IdISSC) is a platform whose objective is to provide scientific and technical support to the different research groups in order to carry out research projects involving the use of flow cytometry in the characterisation and monitoring of different pathologies and biological processes.

It has an FC500 cytometer (2 lasers, 5 colours), a Gallios cytometer (3 lasers, 10 colours) and a Cytoflex cytometer (3 lasers, 13 colours). Two analysis software packages are also available for data processing (KALUZA analysis software and CytExpert analysis software). We are currently in the process of acquiring a sorter.

In addition, due to the increase of companies demanding quality controls, a series of internal and external protocols will be implemented in an attempt to implement the ISO9100 standard.

Opening hours: the equipment will be available from Monday to Friday, from 9:00 to 18:00 h.

+ Info

Dra. Dulcenombre Gómez Garre
mgomezgarre@salud.madrid.org
Tel. +34 91 3303000 Ext. 482455-482456
2nd Floor North
Hospital Clínico San Carlos
C/ Profesor Martín Lagos s/n.
28040 Madrid

Services offered

The services offered by the Cytometry Research Support Transversal Unit (RSTU) to date are:

Sample processing and acquisition for cytometer analysis
Cytometric data analysis
At the request of the investigators, technical assistance will be provided for the study and observation of samples under the best possible conditions, for the design of analytical protocols, the selection of antibodies and the processing and acquisition of data, as well as their analysis.
Conducting training courses for users

Fees in force

Current customers

HCSC:
- - Allergy group
  - Multiple Sclerosis Group
  - Environmental Factors in Degenerative Diseases Group
  - Physiopathology and neurological therapeutics of INA (NEURO-INA-IN) Group
  - Genetics and Molecular Basis of Complex Diseases Group
  - Pharmaceutical Innovation in Ophthalmology Group
  - Clinical and Translational Research in Oncology Group
  - Translational Research in Acute Myocardial Syndromes (INTEGRA) Group
  - Cardiovascular Risk Group
  - Haematology and Haemotherapy Department
  - Endocrinology Clinical Management Unit
  - Immunology Clinical Management Unit
  - Rheumatology Clinical Management Unit
  - Cell Therapy Unit Clean Room
UCM:
- Laboratory of Neurobiology of Hearing (Faculty of Medicine)
- Pharmacology and Toxicology Department (Faculty of Medicine)

Potential customers

Any user requiring identification of cells and/or components by flow cytometry.

The Biobank is attached to 2 external committees:

Relevant publications

- 2020 – Glia – Microglial CX3CR1 productionincreases in Alzheimer’sdisease and isregulatedbynoradrenaline
- 2018 – Plosone. 2018; 5; 13(1): e0190494 – Effect of intensive multifactorial treatmenton vascular progenitor cells in hypertensivepatients. Maroun-Eid C *; Ortega-Hernández A*; Abad-Cardiel A; García-Donaire JA; Reinares L; Kruzliak P; Martell-Claros
- 2018 – DigLiverDis. 50(11):1183-118. – Evaluation of T cells in bloodafter a short gluten challengeforcoeliacdisease diagnosis. López-Palacios N, Pascual V, Castaño M, Bodas A, Fernández-Prieto M, Espino-Paisán L, Martínez-Ojinaga E, Salazar I, Martínez-Curiel R, Rey E, Estrada L, Molero-Abraham M, Reche PA, Dieli-Crimi R, Núñez C.
- 2017 – VetImmunol Immunopathol.190:65-72 – Mite allergoids coupled to nonoxidizedmannan from Saccharomyces cerevisae efficiently target canine dendritic cells for novel allergy immunotherapy in veterinary medicine. Soria I, Alvarez J, Manzano AI, López-Relaño J, Cases B, Mas-Fontao A, Cañada FJ, Fernández-Caldas E, Casanovas M, Jiménez-Barbero J, Palomares O, Viñals-Flórez LM, Subiza JL.
- 2015 – Hipertens Riesgo Vasc 2015;32:142-150 – Circulating endothelial progenitor cell levels in treated hypertensive patients
- 2014 – Circ Res 2014;115:950-60 – Selected CD133(+) Progenitor Cells to Promote Angiogenesis in Patients With Refractory Angina Final Results of the PROGENITOR Randomized Trial

Other members of the Unit

- Dra. Adriana Ortega Hernández
- Dr. Javier Modrego Martin
- Rubén Gómez-Gordo Diaz